Designing a Waste-Based Culture Medium for the Production of Plant Growth Promoting Microorganisms Based on Cladodes Juice from Opuntia ficus-indica Pruning

The production of beneficial microorganisms is the first step to obtain a commercial-based product for application in agriculture. In this study, prickly pear (Opuntia ficus-indica) pruning waste was evaluated as raw material large amounts Plant Growth Promoting Microorganisms (PGPMs) reducing number generated wastes. Specifically, five PGPMs constituting synthetic microbial consortium with complementing plant growth-promoting traits were grown on laboratory scale and, subsequently, pilot using 21-L bioreactor. Primarily, physical-chemical characterization culture medium obtained from juice Opuntia cladodes carried out, revealing presence sugars and organic acids different molar ratios. Compared conventional media, did not show significant differences bacterial growth efficiency. Instead, survival rates bacteria after air-drying zeolite or freeze-drying, significantly higher than those observed when they media. present work conducted pilot-scale that maximizes submerged fermentation Opuntia, both economic environmental impacts associated generation